Product Description
Through extensive research, an intercalating DNA dye has been developed which is suitable for real-time quantification of
amplified DNA without inhibiting the polymerization reaction, often seen with other popular intercalating dyes. Hi-Tech
Green has an optimized buffer mixture, able to efficiently amplify GC- and AT-rich using standard or fast cycling
conditions. Unspecific signal detection and lower amplification efficiency originated from primer-dimers are inhibited
using small molecule inhibition. This ready to use mix can replace any commercial Dye based qPCR mixture. The
annealing temperature may need to be optimized to account for differences in formulation. Hi-Tech Green qPCR mix has
a dye that does not inhibit PCR and it comes with separate ROX vial for addition to the master mix.
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